Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NUDT21

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
H9 cell line
cell line
human embroynic stem cell line H9
chip antibody
10322-1-AP-50ul, Proteintech
genotype
CFIm25 KO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS, and ChIP-seq libraries were prepared using CUT&TagHyperactive In-Situ ChIP Library Prep Kit for Illumina(pG-Tn5)(TD901-01, vazyme) and the indicated antibodies. ChIP-seq libraries were prepared using VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 (vazyme, ND607-01) Libraries were sequenced with Novaseq PE150

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
27450700
Reads aligned (%)
51.1
Duplicates removed (%)
41.4
Number of peaks
144 (qval < 1E-05)

hg19

Number of total reads
27450700
Reads aligned (%)
50.9
Duplicates removed (%)
41.6
Number of peaks
112 (qval < 1E-05)

Base call quality data from DBCLS SRA